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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
Published on: August 25, 2018
Synchronized growth in human epidermis following tape-stripping: its implication for cell kinetic studies
J J Rijzewijk1, J B Boezeman, F W Bauer
1Department of Dermatology, University of Nijmegen, The Netherlands.
Abstract:
A recent investigation of the hyperproliferative activity of human epidermis following sellotape stripping showed a wave of cell divisions with a maximal percentage of cells in mid-S phase at about 39 h after stripping. Here we present a study over the period of 52-76 h following stripping, showing a second wave of cell divisions with a maximal percentage of cells in mid-S at about 63 h. This indicates an average cell cycle time of about 23 h. Human epidermis after tape-stripping provides us with a useful model of synchronized growth, allowing us to study drug influences on cell kinetics accurately.
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