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Inflammatory Factors Induce Thrombosis through the miR-146b-3p/p38MAPK/COX-2 Pathway

Zhengjia Su1, Fang Wu1

  • 1Department of Geratology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Biomed Research International
|April 28, 2020
PubMed
Abstract

Insights

MicroRNA-146b-3p (miR-146b-3p) influences inflammation in arterial thrombosis by regulating the P38MAPK/COX-2 pathway. Downregulation of miR-146b-3p promotes inflammation, suggesting its therapeutic potential.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Inflammation Research

Background:

  • Inflammatory responses are critical in atherosclerosis development.
  • Understanding molecular regulators of inflammation in thrombosis is essential.

Purpose of the Study:

  • To investigate the relationship between microRNA-146b-3p (miR-146b-3p) and inflammatory factors in thrombosis.
  • To elucidate the role of miR-146b-3p in the P38MAPK/COX-2 signaling pathway.

Main Methods:

  • THP-1 cells were utilized for in vitro experiments.
  • Western blot and real-time PCR were employed to assess protein and mRNA expression levels of COX-2, p38MAPK, and miR-146b-3p.
  • Lentiviral vectors were used to manipulate miR-146b-3p expression.

Main Results:

  • Angiotensin II (Ang II) and Tumor Necrosis Factor-alpha (TNF-α) increased COX-2 expression and p38MAPK phosphorylation.
  • miR-146b-3p downregulated COX-2 expression and p38MAPK phosphorylation.
  • TNF-α significantly enhanced miR-146b-3p expression.

Conclusions:

  • Altered miR-146b-3p expression is linked to inflammation progression in thrombosis via the P38MAPK/COX-2 pathway.
  • The miR-146b-3p/p38MAPK/COX-2 axis is a key player in inflammation and arterial thrombosis.

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