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Accurate and specific HPLC assay of coproporphyrinogen III oxidase activity in human peripheral leucocytes
1Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 31, 1988
Abstract:
An HPLC method is described for the assay of coproporphyrinogen III oxidase in human leucocytes. The optimal pH for the assay was 6.5-7.0 and the Km for coproporphyrinogen III was 0.12 +/- 0.021 mumol/l. The mean activity in 28 apparently health subjects was 0.249 (2 SD range 0.130-0.368) nmol/h per mg protein. In two patients with hereditary coproporphyria, the activities were 0.029 and 0.078 nmol/h per mg protein.