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Injectable and Crosslinkable PLGA-Based Microribbons as 3D Macroporous Stem Cell Niche.

Danial Barati1, Kira Watkins2, Zhibin Wang1

  • 1Department of Orthopedic Surgery, Stanford School of Medicine, Stanford, CA, 94305, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|April 28, 2020
PubMed
Summary

Injectable Poly(lactide-co-glycolide) microribbon scaffolds offer a novel 3D stem cell niche. These macroporous scaffolds enable homogeneous cell encapsulation and promote robust bone formation for tissue regeneration.

Keywords:
microribbonspoly(lactide-co-glycolide) (PLGA)scaffoldsstem cell nichestem cellstissue regeneration

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Poly(lactide-co-glycolide) (PLGA) is a common biomaterial for tissue engineering scaffolds.
  • Conventional PLGA scaffolds lack injectability and hinder uniform cell distribution in 3D.
  • There is a need for advanced scaffolds that support cell encapsulation and 3D tissue formation.

Purpose of the Study:

  • To develop injectable and intercrosslinkable Poly(lactide-co-glycolide) microribbon (PLGA μRB) scaffolds.
  • To create a 3D stem cell niche that facilitates homogeneous cell encapsulation and proliferation.
  • To evaluate the potential of these scaffolds for bone tissue regeneration.

Main Methods:

  • Fabrication of PLGA microribbons using microcontact printing.
  • Coating PLGA microribbons with fibrinogen to improve solubility and injectability.
  • Intercrosslinking of microribbons with thrombin to form 3D macroporous scaffolds.
  • Assessment of scaffold mechanical properties under cyclic compression.
  • Encapsulation and culture of human mesenchymal stem cells (hMSCs) within the scaffolds.
  • Evaluation of osteogenic differentiation and bone formation.

Main Results:

  • PLGA μRB scaffolds demonstrated excellent injectability and intercrosslinking capabilities.
  • The scaffolds exhibited superior shock-absorbing capacity and shape recovery compared to conventional PLGA.
  • Homogeneous encapsulation, spreading, and proliferation of hMSCs were observed in 3D.
  • Significant increases in compressive modulus and robust bone formation (alkaline phosphatase, mineralization, collagen) were achieved after 28 days of osteogenic culture.

Conclusions:

  • PLGA microribbons can be fabricated into injectable, macroporous scaffolds for 3D stem cell niches.
  • These novel scaffolds support cell delivery, proliferation, and osteogenic differentiation.
  • PLGA μRB scaffolds represent a promising non-hydrogel-based alternative for tissue regeneration applications.