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Related Concept Videos

Gastrulation01:56

Gastrulation

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Updated: Dec 23, 2025

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

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Using Single-Cell and Spatial Transcriptomes to Understand Stem Cell Lineage Specification During Early Embryo

Guangdun Peng1,2,3, Guizhong Cui2, Jincan Ke1

  • 1CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China;

Annual Review of Genomics and Human Genetics
|April 28, 2020
PubMed
Summary
This summary is machine-generated.

Single-cell RNA sequencing and spatial transcriptomics reveal molecular trajectories in stem cell differentiation and embryonic development. These advanced techniques help map tissue architecture and cell fate determination during gastrulation.

Keywords:
cell lineageembryo developmentsingle-cell genomicsspatial transcriptometrajectory inference

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genomics

Background:

  • Embryonic development and stem cell differentiation are key models for understanding cell fate determination and tissue patterning.
  • Technological advancements are crucial for dissecting complex biological processes at a cellular level.

Purpose of the Study:

  • To review the application of single-cell RNA sequencing in studying stem cell lineages and gene regulatory networks.
  • To highlight the integrated analysis of single-cell and spatial transcriptomics for annotating tissue lineages during embryonic development.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) for analyzing molecular trajectories and gene regulation networks.
  • Spatial transcriptomics for understanding cell organization and tissue architecture.
  • Integrated computational and experimental analysis of transcriptomic data.

Main Results:

  • scRNA-seq enables detailed investigation of molecular pathways in stem cell differentiation.
  • Spatial transcriptomics provides insights into cell positioning and interactions within developing tissues.
  • Combined approaches facilitate comprehensive molecular annotation of developmental lineages.

Conclusions:

  • Advanced transcriptomic technologies are revolutionizing the study of embryonic development and stem cell differentiation.
  • Integrated analysis of single-cell and spatial data is essential for understanding tissue lineage allocation and patterning.
  • These methods offer powerful tools for revisiting fundamental questions in developmental biology.