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Related Experiment Video

Updated: Dec 23, 2025

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Published on: September 9, 2020

Customizable live-cell imaging chambers for multimodal and multiplex fluorescence microscopy.

Adam Tepperman¹, David Jiao Zheng¹, Maria Abou Taka¹

¹Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire

|April 28, 2020

Summary

Researchers developed 3D-printed live-cell imaging chambers for high-throughput microscopy. This inexpensive and customizable method supports various imaging modalities and cell culture applications.

Keywords:

3D printing chemotaxis chimiotaxie imagerie de cellules vivantes impression 3D live-cell imaging microscopie microscopy phagocytose phagocytosis

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Area of Science:

Biotechnology
Microscopy
3D Printing

Background:

High-throughput microscopy research requires specialized imaging chambers.
Custom and commercial chambers are often expensive, inflexible, or difficult to manufacture.

Purpose of the Study:

To demonstrate the use of 3D printing for creating affordable, customized live-cell imaging chambers.
To present a versatile method compatible with multiple microscopy techniques.

Main Methods:

Utilizing biocompatible plastics for 3D printing custom imaging chambers.
Adhering printed chambers to glass coverslips for liquid impermeability.
Designing chambers for specific applications like multiplex microscopy and microfluidics.

Main Results:

Successfully produced inexpensive, customized imaging chambers using 3D printing.
Demonstrated compatibility with various imaging modalities, including super-resolution microscopy.
Validated chamber utility for live-cell imaging over multiple days and diverse experimental setups.

Conclusions:

3D printing offers a cost-effective and highly customizable solution for live-cell imaging chambers.
This approach enhances the throughput and flexibility of microscopy-based research.
The method is adaptable for creating molds for microfluidic devices.