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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Methods in microbiome research: Past, present, and future.

Emily C Gotschlich1, Robert A Colbert2, Tejpal Gill3

  • 1NIH/NIAMS, Building 4 Room 228, 4 Memorial Drive, Bethesda, MD 20892, USA.

Best Practice & Research. Clinical Rheumatology
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PubMed
Summary
This summary is machine-generated.

The human microbiome influences immune system development and function. Dysbiosis, or microbial imbalance, is linked to immune-mediated and autoimmune diseases, highlighting the microbiome

Keywords:
16S rRNA gene sequencingDysbiosisEcological modelMetabolomicsMetagenomicsMicrobiomeMucosal immunityTolerance

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Area of Science:

  • Microbiology
  • Immunology
  • Autoimmune Diseases

Background:

  • The human microbiome plays a crucial role in health and wellness.
  • Microbes educate the immune system, promoting infection fighting and immune tolerance.
  • Loss of immune tolerance to microbiota is implicated in immune-mediated and autoimmune conditions.

Purpose of the Study:

  • To discuss methods for studying the microbiome.
  • To review fundamental concepts in microbiome research.
  • To explore the relationship between the microbiome and rheumatology.

Main Methods:

  • Review of established and novel techniques for microbiome analysis.
  • Discussion of core principles in microbiome research.
  • Exploration of current and future research directions in rheumatology.

Main Results:

  • The human microbiome is integral to immune system development and homeostasis.
  • Microbial dysbiosis contributes to the pathogenesis of autoimmune diseases.
  • Understanding microbiome-immune interactions is key for future therapeutic strategies.

Conclusions:

  • The microbiome significantly impacts immune system function and tolerance.
  • Further research into microbiome-host interactions is essential for understanding and treating autoimmune diseases.
  • Future directions in microbiome research hold promise for rheumatology.