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Updated: Dec 23, 2025
Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature
Ali Rajabi1, Zohreh Sharifi1, Fatemeh Yari1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Objective:
MicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. MiR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. It has been shown that platelets could be stored in Composol for a longer period of time. The aim of the present study was to assess and compare the expression pattern of miR-16 in platelet concentrates (PCs) in plasma and Composol media.
Materials And Methods:
In an experimental study, ten PC bags were collected and each bag was divided into two separate bags, one with the 70% Composol and the other with only plasma. Both bags were stored for 7 days at 22˚C and tested on days 1, 3, 5, and 7 of storage. For each sample, we performed quantitative real-time polymerase chain reaction (qRT-PCR). The water-soluble tetrazolium salt-1 (WST-1) test was used to assess platelet viability in all of the samples. Statistical analysis was done by SPSS and REST software. A P<0.05 was considered statistically significant.
Results:
miR-16 was significantly elevated during the storage days, with fold changes of 3.47 (plasma) and 2.77 (Composol). The Composol group had significantly decreased miR-16 expression compared with the plasma group. Results of the WST-1 test showed less decrease in optical density (OD) in the Composol group (0.93 ± 0.4) during the storage days compared with the plasma group (0.75 ± 0.3).
Conclusion:
Our finding supported results from previous studies that reported an increase in miR-16 expression during platelet storage. In addition, miR-16 down-regulation in Composol medium implied that Composol might be a good solution for long-term platelet storage because it has the potential to elevate the shelf-life of platelets stored at 22˚C.
Insights
Platelet storage in Composol medium showed lower miR-16 expression compared to plasma, suggesting Composol may extend platelet shelf-life. This finding supports Composol as a viable option for long-term platelet concentrate storage.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- MicroRNAs (miRNAs) regulate gene expression and are affected by platelet storage.
- MiR-16, highly expressed in platelets, targets genes involved in cell death.
- Platelets can potentially be stored longer in Composol medium.
Purpose of the Study:
- To compare the expression pattern of miR-16 in platelet concentrates (PCs) stored in plasma versus Composol medium.
- To evaluate the effect of storage media on miR-16 levels in platelets.
Main Methods:
- Ten PC bags were divided into plasma and 70% Composol groups, stored for 7 days at 22°C.
- Quantitative real-time PCR (qRT-PCR) was used to measure miR-16 expression.
- Platelet viability was assessed using the WST-1 test.
Main Results:
- miR-16 expression increased during storage in both groups.
- Composol group showed significantly lower miR-16 expression (2.77-fold) compared to the plasma group (3.47-fold).
- Platelet viability, indicated by WST-1 optical density, was better preserved in the Composol group (0.93 ± 0.4) than in the plasma group (0.75 ± 0.3).
Conclusions:
- Findings confirm increased miR-16 during platelet storage.
- Down-regulation of miR-16 in Composol suggests its potential for extending platelet shelf-life.
- Composol may be a suitable medium for long-term storage of platelets at 22°C.
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