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Temporal-Spatial-Color Multiresolved Chemiluminescence Imaging for Multiplex Immunoassays Using a Smartphone Coupled
1Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui 230009, P.R. China.
Analytical Chemistry
|April 30, 2020
Summary
This study introduces a novel temporal-spatial-color imaging strategy for multiplex label-free immunoassays. This method enables sensitive, simultaneous detection of multiple cancer biomarkers using a smartphone and microfluidic chip.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Nanotechnology
Background:
- Chemiluminescence (CL) bioassays are crucial for clinical diagnosis.
- Multiplex analysis for simultaneous biomarker detection on a single chip remains challenging for rapid disease diagnosis.
Purpose of the Study:
- To develop a novel temporal-spatial-color multiresolved CL imaging strategy.
- To enable multiplex, label-free immunoassays for sensitive and simultaneous biomarker detection.
Main Methods:
- Utilized a smartphone coupled with a microfluidic chip for CL imaging.
- Employed ZIF-67 catalyzed luminol-H2O2 CL and CL resonance energy transfer reactions.
- Sequentially transported coreactant H2O2 to detection zones for signal generation.
Main Results:
- Achieved multiplex analysis of three model cancer biomarkers.
- Demonstrated low detection limits in the pg/mL to fg/mL range.
- Exhibited good selectivity and low cost, avoiding complex labeling.
Conclusions:
- The developed strategy offers a promising approach for multiplex, label-free immunoassays.
- This work advances CL imaging for sensitive and simultaneous disease biomarker analysis.
- Opens new avenues for temporal-spatial-color multiresolved imaging in diagnostics.
