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[Specificity of mutagenic effect of N-nitroso-N-methylurea and relation between its mutagenic activity and
Abstract:
Significance of carbamoylation for mutagenic effects of N-nitroso-N-methyl-urea (NMU) on the CHO-AT3-2 cell line of Chinese hamster was studied. True point mutations occurred, due to alkylation. Carbamoylation combined with alkylation, or carbamoylation after alkylation induced the increase in other types of gene mutations as well as micro- and macroaberrations. These effects may be explained by the synergistic effect of alkylation and carbamoylation. Possible mechanisms and levels of interaction between alkylation and carbamoylation are discussed.
Insights
Carbamoylation enhances the mutagenic effects of N-nitroso-N-methyl-urea (NMU) on Chinese hamster cells. This combination increases gene mutations and chromosomal aberrations, suggesting a synergistic interaction.
Area of Science:
- Molecular toxicology
- Genetics
- Cell biology