Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Timothy syndrome and CACNA1C-Related Disorder: first international language and management guidelines consensus statement.

European journal of human genetics : EJHG·2026
Same author

A two-tier framework for responsible research on human embryo models.

Cell·2026
Same author

Biomarker innovations in precision psychiatry diagnostics and treatment strategies.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology·2026
Same author

Timothy Syndrome and CACNA1C-Related Disorder: First International Language and Management Guidelines Consensus Statement.

Research square·2025
Same author

Human embryo research: how to move towards a 28-day limit.

Nature·2025
Same author

Safeguarding genomic imprints in naive human pluripotency.

Stem cell reports·2025

Related Experiment Video

Updated: Dec 22, 2025

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.3K

Benchmarking pluripotent stem cell-derived organoid models.

Alejandro De Los Angeles1, Elizabeth M Tunbridge2

  • 1Department of Psychiatry, Warneford Hospital, University of Oxford, Oxford OX3 7JX, United Kingdom.

Experimental Neurology
|May 1, 2020
PubMed
Summary
This summary is machine-generated.

Human brain organoids partially mimic brain development but lack cell subtypes and show stress markers. Improving these models requires addressing in vitro culture limitations for accurate research.

More Related Videos

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
10:16

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

6.4K
Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
06:34

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

3.3K

Related Experiment Videos

Last Updated: Dec 22, 2025

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.3K
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
10:16

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

6.4K
Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
06:34

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

3.3K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Stem Cell Research

Background:

  • Cerebral organoids are 3D stem cell cultures that model human brain development.
  • They show promise for research and drug development due to cellular diversity.
  • Questions remain regarding their fidelity to in vivo human neurodevelopment.

Purpose of the Study:

  • To assess the accuracy of cerebral organoid models in recapitulating human brain development.
  • To compare the molecular profiles of organoid cells with primary fetal brain cells.

Main Methods:

  • Comparison of molecular profiles between cerebral organoid cells and primary fetal brain cells.
  • Analysis of cellular diversity and stress marker expression in organoids.
  • In vivo transplantation of organoid cells into neonatal mouse brains.

Main Results:

  • Brain organoids broadly replicate the cellular profile of the human brain.
  • Organoids lack specific subtypes of cell classes found in the human brain.
  • Significant expression of cellular stress markers was observed in organoids, which decreased after transplantation.

Conclusions:

  • In vitro culture conditions may induce cellular stress, impairing organoid maturation.
  • Cerebral organoids offer a foundation for future model improvement but require caution for early human brain development studies.
  • Further research is needed to enhance the accuracy of organoid models.