Effect of oxLDL on transcriptional expression of human lens epithelial cells

Age-related cataract patients regularly have hypertension, hyperglycemia, and hyperlipidemia. In oxidative conditions, increased reactive oxygen species can oxidize natural low-density lipoprotein into oxidative low-density lipoprotein (oxLDL). However, the relationship between oxLDL and the occurrence of cataracts is still unclear. In this study, 1515 differentially expressed transcripts were identified by analyzing the results of RNA sequencing in a human lens epithelial cell line (HLEpiC). Compared with control groups, oxLDL-treated HLEpiC had 806 up-regulated transcripts and 709 down-regulated transcripts. Our genome-wide transcriptome results showed that differentially expressed genes, such as Rho signaling (Rho A and Cdc42) and Na⁺/K⁺-ATPase family (ATP1B1), are involved in lens epithelial cell differentiation and cell homeostasis. In conclusion, oxLDL greatly influences transcriptional expression and these differentially expressed genes may play an important role in the development of cataracts. Our findings may provide new targets in the treatment for cataracts.