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Determination of pterins in urine by high-performance liquid chromatography on C18 columns conditioned with
B C Montalvo1, C I Villar, R C Hornillos
1Facultad de Ciencias Quimicas, Departamento de Quimica Analítica, Universidad Complutense de Madrid, Spain.
Journal of Chromatography
|December 23, 1988
Abstract:
A method is proposed for the determination of pterins in urine without clean-up by reversed-phase high-performance liquid chromatography on a C18 column previously conditioned with cetyltrimethylammonium bromide. Other endogenous compounds are retained in the column, preventing interference. Xanthopterin, neopterin, isoxanthopterin, monapterin, biopterin, 6-hydroxymethylpterin, pterin and lumazine can be determined. Retention and separation mechanisms are discussed.