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Self-Assembling Behavior of pH-Responsive Peptide A6K without End-Capping.

Peng Zhang1, Fenghuan Wang1, Yuxuan Wang1

  • 1School of Light Industry, Beijing Technology and Business University (BTBU), Beijing 100048, China.

Molecules (Basel, Switzerland)
|May 3, 2020
PubMed
Summary

This study designed a pH-responsive peptide, A6K, that self-assembles into different nanostructures like blocks and nanoribbons based on solution pH. These findings advance the design of novel pH-responsive peptide-based nanomaterials.

Keywords:
molecular dynamics simulationnanoribbonpH-responsive peptideself-assemblyβ-turn

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Peptide Chemistry

Background:

  • Self-assembling peptides offer versatile platforms for nanomaterial development.
  • Understanding peptide behavior across varying pH is crucial for controlled assembly.

Purpose of the Study:

  • To design and characterize a short, pH-responsive self-assembling peptide (A6K).
  • To investigate the influence of pH on the self-assembly behavior and resulting nanostructures of A6K.
  • To elucidate the structural basis of A6K self-assembly using computational and experimental methods.

Main Methods:

  • Computer simulation for peptide charge distribution modeling.
  • Transmission electron microscopy (TEM) for observing self-assembled structures.
  • Circular dichroism (CD) spectroscopy for secondary structure analysis.
  • Molecular dynamics (MD) simulations for structural insights.

Main Results:

  • A6K peptide exhibits pH-responsive self-assembly into distinct structures (blocks and nanoribbons).
  • Block structures are associated with random coil conformations.
  • Nanoribbon formation correlates with increased beta-turn content.
  • Electrostatic interactions significantly influence self-assembly pathways.

Conclusions:

  • A6K is a pH-responsive peptide capable of forming diverse nanostructures.
  • Secondary structure transitions (random coil to beta-turn) dictate the assembly outcome.
  • This research provides a foundation for designing advanced pH-responsive peptide-based nanomaterials.