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Updated: Dec 22, 2025

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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
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New primers, taxonomic database and cut-off value for processing nxrB gene high-throughput sequencing data by MOTHUR
Yiguo Hong1, Lijing Jiao1, Jiapeng Wu1
1Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Institute of Environmental Research at Greater Bay, Guangzhou University, Guangzhou 510006, PR China.
Journal of Microbiological Methods
|May 4, 2020
Abstract:
To analyse the diversity of Nitrospira with functional nxrB genes, we designed and optimized a new primer set, Nxr-f27/Nxr-r617, and constructed a database for taxonomic classification. We also propose a cut-off value for processing the high-throughput sequencing data with MOTHUR.

