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Polydopamine Nanosphere with In-Situ Loaded Gentamicin and Its Antimicrobial Activity.
Rahila Batul1, Mrinal Bhave1, Peter J Mahon1
1Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Molecules (Basel, Switzerland)
|May 6, 2020
Summary
Polydopamine nanoparticles (PDA NPs) effectively loaded the antibiotic gentamicin, showing promise for long-term microbial infection treatment. The 1:1 drug-to-monomer ratio achieved the highest loading capacity and antibacterial activity.
Area of Science:
- Nanomedicine
- Materials Science
- Biotechnology
Background:
- Mussel-inspired polydopamine (PDA) exhibits excellent physicochemical properties for nanomedicine applications.
- Polydopamine nanoparticles (PDA NPs) offer low cytotoxicity, high biocompatibility, and biodegradability, making them suitable for drug delivery.
- PDA NPs possess a high capacity for loading drugs and chemotherapeutics due to their reactive nature.
Purpose of the Study:
- To investigate the loading efficiency of PDA NPs for the antibiotic gentamicin (G).
- To optimize the drug-to-monomer ratio for maximum gentamicin loading into PDA NPs.
- To evaluate the antibacterial efficacy of gentamicin-loaded PDA NPs against Gram-positive and Gram-negative bacteria.
Main Methods:
- An in-situ polymerization method was employed to synthesize gentamicin-loaded PDA NPs.
- Variable drug-to-monomer ratios were used to determine optimal loading conditions.
- Characterization techniques included SEM, FTIR, and XPS to confirm drug loading.
- Liquid chromatography-mass spectrometry (LC-MS) quantified the loading amount.
- Antibacterial activity was assessed using the broth microdilution method.
Main Results:
- Successful loading of gentamicin into PDA NPs was confirmed by SEM, FTIR, and XPS, primarily through hydrogen bonding.
- The highest loading capacity was achieved with a drug-to-monomer ratio of 1:1 (G-PDA).
- The G-PDA sample with the highest loading exhibited the lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values.
- Effective antibacterial activity was observed against both *Staphylococcus aureus* and *Pseudomonas aeruginosa*.
Conclusions:
- The developed gentamicin-loaded PDA NPs demonstrate significant potential for long-term drug release.
- These nanoparticles show promise for effectively treating various microbial infections.
- The study highlights the efficacy of PDA NPs as a drug delivery system for antibiotics.
