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Ibuprofen: Fish Short-Term Reproduction Assay with Zebrafish (Danio rerio) Based on an Extended OECD 229 Protocol.
Lisa A Constantine1, John W Green2, Suzanne Z Schneider3
1Pfizer Worldwide Research and Development, Groton, Connecticut, USA.
Environmental Toxicology and Chemistry
|May 6, 2020
Summary
Ibuprofen does not disrupt fish reproduction. This study found no endocrine-mediated reproductive effects in zebrafish exposed to environmentally relevant ibuprofen concentrations.
Area of Science:
- Environmental Toxicology
- Endocrinology
- Aquatic Toxicology
Background:
- Ibuprofen is a common pharmaceutical pollutant.
- Potential endocrine disruption in aquatic organisms is a concern.
Purpose of the Study:
- To assess ibuprofen's impact on the fish reproductive axis.
- To determine endocrine-mediated reproductive effects in zebrafish.
Main Methods:
- Fish Short-Term Reproduction Assay (OECD 229) adapted for zebrafish.
- Exposure to nominal ibuprofen concentrations (0.5–265.4 µg/L) and control.
- Evaluation of F0 generation reproduction and F1 generation hatching success.
Main Results:
- No-observed-effect concentration (NOEC) and lowest-observed-effect concentration (LOEC) determined.
- No significant impact on fecundity, fertility, or hatching success observed.
- Vitellogenin levels in F0 generation remained unaffected.
Conclusions:
- Ibuprofen does not cause endocrine-mediated reproductive disruption in zebrafish.
- Findings suggest safety at environmentally relevant concentrations.
