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Related Concept Videos

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CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
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Uricase-deficient rat is generated with CRISPR/Cas9 technique.

Yun Yu1, Nan Zhang1, Xianxiang Dong1

  • 1The Department of Pharmacology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan, China.

Peerj
|May 6, 2020
PubMed
Summary

Scientists created uricase-deficient rats, named Kunming-DY rats, to model hyperuricemia. These rats are healthy and offer a stable alternative for studying conditions like gout.

Keywords:
CRISPR/Cas9 techniqueHigh throughput sequencingHyperuricemiaLipid metabolismModel animalRenal functionUricase-deficiency

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Area of Science:

  • Biomedical Research
  • Genetics and Genomics
  • Animal Models

Background:

  • Urate oxidase (uricase) deficiency is a significant barrier in creating animal models for hyperuricemia research.
  • Existing uricase-deficient mouse models have low survival rates, limiting their utility.

Purpose of the Study:

  • To establish a stable, viable animal model for studying hyperuricemia and related disorders.
  • To overcome the limitations of current uricase-deficient models.

Main Methods:

  • Utilized CRISPR/Cas9 gene-editing technology to delete exons 2-4 of the Uox gene in Sprague Dawley rats.
  • Generated a novel line of uricase-deficient rats, named Kunming-DY rats.

Main Results:

  • Kunming-DY rats exhibited apparent health and over 95% survival rate up to one year.
  • Significantly elevated serum uric acid levels were observed in male rats compared to wild-type.
  • While some blood fat and renal function indexes differed, they remained within normal ranges; mild renal histological changes were noted.

Conclusions:

  • Successfully established Kunming-DY rats as a stable, viable model for uricase deficiency.
  • This new rat model serves as a valuable alternative for studying hyperuricemia and associated human diseases.