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Generation of RUNX1-null reporter human embryonic stem cell line GIBHe008-A

Hanif Ullah1, Hao You2, Zahir Shah1

  • 1Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Stem Cell Research
|May 7, 2020
PubMed

Keywords:
Gene targetingHuman embryonic stem cells (hESCs)In vitro differentiationRUNX1TALEN

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...