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Related Concept Videos

DNA Isolation01:34

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Related Experiment Video

Updated: Dec 22, 2025

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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Construction of a system for single-stranded DNA isolation.

Min Hao1,2,3, Huanbang Huang1,2,3, Yasai Hu1,2,3

  • 1School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Biotechnology Letters
|May 7, 2020
PubMed
Summary

A new Strep-Tactin and StrepII tag-SSB protein binding (ST-SSB) system efficiently isolates single-stranded DNA (ssDNA) in one step. This cost-effective method purifies ssDNA from mixed samples with high recovery rates.

Keywords:
IsolationST-SSB systemSingle-stranded DNAStrepII tag-EcSSB protein

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Biotechnology

Background:

  • Isolating single-stranded DNA (ssDNA) is crucial for various molecular biology applications.
  • Existing methods for ssDNA purification can be time-consuming, costly, or inefficient.

Purpose of the Study:

  • To establish a novel, efficient, and cost-effective system for single-step purification of ssDNA.
  • To evaluate the performance of the Strep-Tactin and StrepII tag-SSB protein binding (ST-SSB) system.

Main Methods:

  • Development of the ST-SSB system utilizing the affinity between Strep-Tactin, StrepII tag-SSB protein, and ssDNA.
  • Testing the system's ability to isolate ssDNA from mixed DNA samples and asymmetric polymerase chain reaction (aPCR) products.

Main Results:

  • The ST-SSB system demonstrated stronger ssDNA binding specificity compared to gene-5-protein (g5p) in the presence of double-stranded DNA (dsDNA).
  • Successfully purified ssDNA from mixed DNA samples and aPCR products within one hour.
  • Achieved an approximate 60% recovery efficiency for purified ssDNA.

Conclusions:

  • The ST-SSB system offers a highly efficient one-step purification method for ssDNA.
  • This system provides a significant advantage for recycling and purifying any ssDNA with improved speed and cost-effectiveness.