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Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension

Rasmus Hejlesen1, Ernst-Martin Füchtbauer1

  • 1Department of Molecular Biology & Genetics, Aarhus University, C.F. Møllers Allé 3, 8000 Aarhus C, Denmark.

Biotechniques
|May 7, 2020
PubMed

Keywords:
donor template generationgene editingmultiple site-directed mutagenesisprolonged overlap extensionseamless cloningseamless plasmid assembly

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