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Updated: Dec 22, 2025

Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic
Published on: November 7, 2020
A cautionary tale of false-negative nasopharyngeal COVID-19 testing
Sean S M Bullis1, Jessica W Crothers2, Shawn Wayne1
1University of Vermont Medical Center, Burlington, VT, United States.
Abstract:
There remains diagnostic uncertainty regarding the sensitivity of reverse transcription polymerase chain reaction in detection of SARS-CoV-2 from nasopharyngeal specimens. We present a case where two nasopharyngeal specimens were negative, followed by a positive sputum sample. Serial testing for COVID-19 is indicated in patients with high pretest probability of disease.
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