Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.1K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.7K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.7K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.2K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.2K
Types Of Column Chromatography01:29

Types Of Column Chromatography

13.0K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
13.0K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

722
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
722
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

8.4K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.

Analytical chemistry·2026
Same author

Integrating mass spectrometry with Nanopore direct RNA sequencing for <i>de novo</i> modification profiling of bacteriophage MS2.

bioRxiv : the preprint server for biology·2026
Same author

RNase 4 improves bottom-up modification mapping of <i>E. coli</i> total tRNAs using HILIC-MS/MS.

bioRxiv : the preprint server for biology·2026
Same author

Utilizing Venturi effect for automated high-throughput droplet-MS from well plates.

The Analyst·2026
Same author

Rapid analysis of terpenes produced by fermentation using flow injection analysis coupled to APCI MS.

Analytical methods : advancing methods and applications·2026
Same author

Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.

ACS measurement science au·2025

Related Experiment Video

Updated: Dec 22, 2025

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.4K

Liquid chromatography above 20,000 PSI.

Matthew J Sorensen1, Brady G Anderson1, Robert T Kennedy1,2

  • 1Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA.

Trends in Analytical Chemistry : TRAC
|May 9, 2020
PubMed
Summary
This summary is machine-generated.

Ultrahigh pressure liquid chromatography (UHPLC) enables faster separations using sub-2 μm particles. This review covers advancements in liquid chromatography (LC) systems operating above 20,000 psi.

Keywords:
Column packingLong columnsOmicsSmall particlesUltrahigh pressure LC

More Related Videos

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions
08:42

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

Published on: October 10, 2014

11.9K
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2&#8217;-deoxyguanosine, in Cultured Cells and Animal Tissues
14:12

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

27.5K

Related Experiment Videos

Last Updated: Dec 22, 2025

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.4K
High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions
08:42

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

Published on: October 10, 2014

11.9K
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2&#8217;-deoxyguanosine, in Cultured Cells and Animal Tissues
14:12

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

27.5K

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Continued improvements in High-Performance Liquid Chromatography (HPLC) have led to faster and more efficient separations.
  • The advent of ultrahigh pressure LC (UHPLC) and commercial instrumentation capable of up to 20,000 psi (pounds per square inch) has enabled rapid separations using sub-2 μm particles.

Purpose of the Study:

  • To review recent advancements and applications in liquid chromatography (LC) operating above 20,000 psi.
  • To discuss the theory, advantages, instrument hardware, column packing, and stationary phase considerations for LC systems exceeding 20,000 psi.

Main Methods:

  • Review of recent literature on high-pressure LC systems and applications.
  • Discussion of instrument hardware and design for pressures exceeding 20,000 psi.
  • Overview of column packing procedures and stationary phase considerations for ultrahigh pressure LC.

Main Results:

  • Instruments capable of operating above 20,000 psi allow for significantly faster and more efficient separations.
  • Specific advancements in hardware, column technology, and packing procedures facilitate these high-pressure separations.
  • High-pressure LC systems have been successfully applied to the analysis of complex mixtures.

Conclusions:

  • Operating LC systems above 20,000 psi offers substantial benefits for chromatographic separations.
  • Further research and development in instrumentation and column technology are crucial for advancing ultrahigh pressure LC.
  • High-pressure LC is a powerful technique for analyzing complex samples efficiently.