Related Experiment Video
Updated: Dec 22, 2025
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7
Jie Li1, Gaofu Wang1, Jing Jiang1
1Herbivorous Livestock Institute, Chongqing Academy of Animal Sciences, Chongqing, 402460, China.
Objective:
MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation.
Methods:
The detailed function of miR-127-3p in proliferative C2C12 cell lines and further identified its regulatory mechanism by qRT-PCR, western blot, flow cytometry analysis and luciferase reporter assay.
Results:
Overexpression of miR-127-3p significantly inhibited proliferation of C2C12 cells and vice versa. Sept7 was a target gene of miR-127-3p using dual-luciferase reporter assay, qRT-PCR, and western blotting. The RNA interference analysis, in which Sept7 was downregulated, showed that Sept7 significantly promoted the proliferation of C2C12 cells. Besides, the expression level of Sept7 was detected analysis in muscle cells and tissues.
Conclusions:
These findings reveal that miR-127-3p regulates myoblast proliferation by targeting Sept7.
Insights
MicroRNA-127-3p (miR-127-3p) inhibits myoblast proliferation by targeting Sept7. This study elucidates the role of miR-127-3p in regulating cell growth via the Sept7 gene.
Area of Science:
- Molecular Biology
- Cell Biology
Background:
- MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally.
- miR-127 is crucial for myogenic differentiation but its role in proliferation is unclear.
Purpose of the Study:
- Investigate the function of miR-127-3p in C2C12 myoblast proliferation.
- Identify the regulatory mechanism of miR-127-3p in myogenesis.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) and western blotting.
- Flow cytometry analysis and dual-luciferase reporter assay.
- RNA interference for Sept7 gene silencing.
Main Results:
- Overexpression of miR-127-3p inhibited C2C12 cell proliferation; conversely, its inhibition promoted proliferation.
- Sept7 was identified as a direct target gene of miR-127-3p.
- Downregulation of Sept7 promoted C2C12 cell proliferation, and Sept7 expression was detected in muscle tissues.
Conclusions:
- miR-127-3p negatively regulates myoblast proliferation.
- Sept7 acts as a key mediator in miR-127-3p's regulation of myoblast proliferation.
More Related Videos
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs
01:13Formation of Muscle Fibers from Myoblasts
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
02:23Master Transcription Regulators
01:02Role of Septins
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
02:23Abnormal Proliferation