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Related Experiment Videos

Flow Rate Affects Nanoparticle Uptake into Endothelial Cells.

Yih Yang Chen1,2, Abdullah Muhammad Syed1,2, Presley MacMillan1,2,3

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, M5S 3G9, Canada.

Advanced Materials (Deerfield Beach, Fla.)
|May 9, 2020
PubMed
Summary

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This summary is machine-generated.

High blood flow velocity decreases nanoparticle uptake by endothelial cells. Surface modification of nanoparticles with binding ligands can restore uptake, guiding future medical applications.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Systemic injection exposes nanoparticles to blood flow, influencing their interaction with endothelial cells.
  • Characterizing the flow-dependence of nanoparticle uptake in vivo is challenging.
  • Endothelial cells lining blood vessels are key sites for nanoparticle interaction and uptake.

Purpose of the Study:

  • To develop a microfluidic system to control nanoparticle flow rates and study their interaction with endothelial cells.
  • To quantify the effect of varying flow rates on gold nanoparticle uptake by endothelial cells.
  • To investigate strategies for mitigating flow-induced reduction in nanoparticle uptake.

Main Methods:

  • Development of a microfluidic device to precisely control nanoparticle flow rates.
Keywords:
blood vesselsflow shearflow velocitymicrofluidicsnanomedicinenanoparticles

Related Experiment Videos

  • Quantification of gold nanoparticle uptake into endothelial cells under defined flow conditions.
  • Assessment of nanoparticle uptake after surface modification with endothelial-cell-binding ligands.

    • Main Results:

    • Increased nanoparticle flow rates significantly decreased endothelial cell uptake.
    • Higher shear rates experienced by endothelial cells correlated with reduced nanoparticle uptake.
    • Surface functionalization with binding ligands partially restored nanoparticle uptake, counteracting flow effects.

    Conclusions:

    • Endothelial cell nanoparticle uptake is highly sensitive to blood flow dynamics.
    • Surface engineering of nanoparticles can enhance their efficacy by overcoming flow-related barriers.
    • The microfluidic system provides a platform for future studies on nanoparticle-endothelial cell interactions under flow, aiding in the design of nanoparticles for medical applications.