Jove
Visualize
Contact Us

Related Experiment Videos

Chromatin subunits from baker's yeast: isolation and partial characterization.

D A Nelson, W R Beltz, R L Rill

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1977
    PubMed
    Summary

    Baker's yeast chromatin structure resembles higher eukaryotes, with DNA organized into nucleosomes. Yeast nucleosomes contain histones similar to those in other organisms, but with a shorter DNA repeat length.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Factors Associated With Testicular Cancer Risk Among New American Military Recruits During Wartime.

    American journal of men's health·2026
    Same author

    Single-Cell Transcriptomic Analysis of Salivary Gland Endothelial Cells.

    Journal of dental research·2024
    Same author

    Comparing functional outcomes in minimally invasive versus open inguinal hernia repair using the army physical fitness test.

    Hernia : the journal of hernias and abdominal wall surgery·2022
    Same author

    Hybrid balloon valvuloplasty for management of severe mitral stenosis in four symptomatic, adult dogs.

    Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2022
    Same author

    Risk factors for musculoskeletal-related occupational disability among US Army soldiers.

    BMJ military health·2021
    Same author

    Regional Differences following Partial Salivary Gland Resection.

    Journal of dental research·2019
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Chromatin, the complex of DNA and proteins, forms the structural basis of eukaryotic genomes.
    • Understanding chromatin organization in model organisms like Saccharomyces cerevisiae provides insights into fundamental biological processes.

    Purpose of the Study:

    • To investigate the structural organization of proteins along DNA in Saccharomyces cerevisiae chromatin.
    • To compare yeast chromatin structure with that of higher eukaryotes.

    Main Methods:

    • Digestion of yeast nuclei with staphylococcal nuclease to obtain DNA and nucleoprotein products.
    • Isolation and resolution of nucleoprotein fragments using linear sucrose gradient centrifugation.
    • Analysis of DNA fragment size and associated proteins via electrophoresis.

    Related Experiment Videos

    Main Results:

    • Yeast DNA is cleaved at regular intervals, approximately 160 base pairs apart.
    • The predominant nucleoprotein fraction (11S) contains DNA of 150-160 base pairs and proteins similar to histones H2A, H2B, H3, and H4.
    • Yeast chromatin exhibits a structural organization comparable to higher eukaryotes, with a shorter DNA repeat length and a lack of cysteine in subunit proteins.

    Conclusions:

    • The fundamental organization of the yeast genome into nucleosomes is conserved across eukaryotes.
    • Saccharomyces cerevisiae serves as a valuable model for studying chromatin structure and function.
    • Differences in DNA repeat length and protein composition highlight evolutionary variations in chromatin organization.