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Updated: Dec 22, 2025
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
Radiosensitivity Effect of Epidermal Growth Factor Receptor Nanoparticles on Head and Neck Squamous Cell Carcinoma
Yaqian Han1, Feng Liu1, Yujuan Zhou1
1Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, PR China.
Abstract:
The effect of antisense epidermal growth factor receptor (EGFR) nanoparticles on the radiosensitivity of squamous cell carcinoma in mice was studied. Antisense EGFR oligonucleotide nanoparticles were introduced into the SCCVII cell line, and the expression of the antisense EGFR in the SCCVII cell line was detected using western blot analysis. After radiotherapy intervention, a cell cloning test and MTT test were used to detect the radiosensitivity of the cells, and flow cytometry was used to detect the cell cycle distribution and apoptosis to establish a model of head and neck cancer in mice. Antisense EGFR nanoparticles were injected into the tumors and treated with 4 Gy radiation therapy to observe their inhibition on the growth of tumors. SCCVII cell line nanoparticles could significantly inhibit the expression of antisense EGFR proteins. The combination of antisense EGFR nanoparticles and radiotherapy could reduce the differentiation and division ability of cancer cells (P < 0.05). It could arrest cancer cells in the G1 phase and significantly increase the apoptotic rate (P <0.05). The growth of tumors in the antisense EGFR nanoparticles group was significantly delayed. Antisense EGFR nanoparticles could induce G1 phase arrest by down-regulating the expression of EGFRs, which had a radiosensitization effect.
Insights
Antisense epidermal growth factor receptor (EGFR) nanoparticles enhance cancer radiotherapy. These nanoparticles, when combined with radiation, significantly inhibit tumor growth and increase cancer cell death.
Area of Science:
- Oncology
- Nanotechnology
- Molecular Biology
Background:
- Squamous cell carcinoma (SCC) is a prevalent cancer type.
- Epidermal growth factor receptor (EGFR) plays a crucial role in SCC proliferation.
- Targeting EGFR is a potential strategy for cancer therapy.
Purpose of the Study:
- To investigate the radiosensitizing effect of antisense EGFR nanoparticles on SCC.
- To evaluate the impact of these nanoparticles on tumor growth and cancer cell characteristics.
Main Methods:
- SCCVII cell line and a mouse model of head and neck cancer were used.
- Western blot analysis detected antisense EGFR expression.
- Cell cloning, MTT assays, and flow cytometry assessed radiosensitivity, cell cycle, and apoptosis.
- Tumor growth inhibition was measured after nanoparticle injection and radiotherapy.
Main Results:
- Antisense EGFR nanoparticles significantly inhibited EGFR protein expression.
- The combination therapy reduced cancer cell differentiation and division (P < 0.05).
- Cancer cells were arrested in the G1 phase, and apoptosis significantly increased (P < 0.05).
- Tumor growth was significantly delayed in the nanoparticle treatment group.
Conclusions:
- Antisense EGFR nanoparticles demonstrate a radiosensitizing effect on SCC.
- Down-regulation of EGFR by these nanoparticles induces G1 phase arrest and enhances radiotherapy efficacy.
- This approach shows promise for improving head and neck cancer treatment.
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