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Effect of MIPU1 gene interference on biological behavior of U251 cell line
Meidong Liu1, Zhi Wang2, Hui Sun2
1Department of Pathophysiology, Xiangya School of Medcine, Central South University, Changsha 410078, China liumd2005@126.com.
Objectives:
To investigate effect of MIPU1 silence on proliferation, apoptosis, migration and invasion in U251 cells.
Methods:
The shRNA recombinant plasmids targeting MIPU1 gene was transfected into U251 cells. Western blotting was used to identify the inhibitory efficiency at 72 h after transfection. The cell viability was measured by MTT colorimetric assay. Hoechest staining and caspase-3 activity were used to detect apoptosis. Then wound healing assay and transwell migration assay were applied to detect the migration and invasion of cells.
Results:
The expression of MIPU1 protein was effectively knocked down in transfected cells (P<0.05). The cellular proliferation was obviously inhibited and apoptosis was increased in shRNA-transfected MIPU1 cells (all P<0.05). The migration and invasion ability of cells transfected with positive plasmid was lower than that in the control group (P<0.05).
Conclusions:
Down-regulation of MIPU1 can promote apoptosis while inhibit the proliferation, invasion, and migration of U251 cells.
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