Related Experiment Video
Updated: Dec 22, 2025

The Soft Agar Colony Formation Assay
Published on: October 27, 2014
High-mobility group box 3 (HMGB3) silencing inhibits non-small cell lung cancer development through regulating
Yunjing Li1, Yongfu Ma1, Tong Zhang1
1Department of Thoracic Surgery, PLA General Hospital, Beijing 100853, China.
Abstract:
It has been reported that high-mobility group box 3 is overexpressed in various cancers. This study aimed to explore its function in non-small cell lung cancer (NSCLC). A546 and H460 cell lines were used for in vivo experiments, scratch healing tests, transwell migration and invasion experiments. It was first found that HMGB3 was highly expressed in tumor tissues in the patients and associated with NSCLC stage. Silencing of HMGB3 significantly slowed the growth, proliferation and invasion of NSCLC in vitro, and repressed cell growth in vivo. Mechanistic studies suggest that the observed effects were mediated by inhibiting the expression of β-catenin/MMP7/c-Myc in Wnt pathway. Our study highlights the role of HMGB3 in NSCLC, which may provide a therapeutic target for the treatment of NSCLC.
Related Concept Videos
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathways
Inhibition of Cdk Activity
Hedgehog Signaling Pathway
MicroRNAs
MicroRNAs
