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Updated: Dec 21, 2025
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
An activity-based two-photon fluorescent probe for real-time and reversible imaging of oxidative stress in the rat
Chunjing Liang1, Xianghan Chen, Qiao Tang
1Beijing National Laboratory for Molecular Science, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of chemistry, Chinese Academy of Sciences, Beijing 100190, China. mingwang@iccas.ac.cn.
Abstract:
The real-time and reversible detection of cellular glutathione and oxidative stress challenges the study of the redox homeostasis of biological systems. We report herein a modular approach to design the Michael addition between glutathione and coumarin derivatives for fluorescence imaging of the reversible and dynamic change of oxidative stress in living cells and the rat brain.
