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An endoplasmic reticulum-targeting fluorescent probe for imaging ˙OH in living cells

Yanyan Zhao1, Hongyu Li, Ziyin Chai

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. shiwen@iccas.ac.cn mahm@iccas.ac.cn.

Chemical Communications (Cambridge, England)
|May 12, 2020
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...