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Matrix metalloproteinase 9-activatable peptide-conjugated hydrogel-based fluorogenic intraocular-lens sensor.

Moo-Kwang Shin1, Yong Woo Ji2, Chae-Eun Moon3

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A novel implantable sensor system using a fluorogenic intraocular lens (FIOL) can detect matrix-metalloproteinase-9, a biomarker for central nervous system (CNS) and eye disorders, enabling early diagnosis through liquid biopsy.

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Area of Science:

  • Biomedical Engineering
  • Ophthalmology
  • Neuroscience
  • Biomaterials Science

Background:

  • The eye's aqueous humor (AH) offers non-invasive access to biomarkers indicative of central nervous system (CNS) disorders.
  • There is a need for advanced implantable devices for continuous intraocular monitoring of these biomarkers.
  • Matrix-metalloproteinase-9 (MMP-9) is a key biomarker associated with degenerative CNS and ocular diseases.

Purpose of the Study:

  • To develop a novel intraocular lens (IOL)-based sensing system for detecting AH biomarkers.
  • To create a biocompatible, enzyme-activatable fluorogenic hydrogel sensor for MMP-9 detection.
  • To demonstrate the clinical feasibility of the sensor for intraocular fluid liquid biopsy.

Main Methods:

  • Development of a peptide-probe-incorporated fluorogenic IOL (FIOL) using poly(ethyleneglycol) diacrylamide (PEGDAAm) hydrogels.
  • Tuning hydrogel mesh size for selective biomarker penetration and non-target blocking.
  • Utilizing label-free detection and conducting in vivo testing to assess clinical feasibility.

Main Results:

  • Successful development of a FIOL system capable of detecting MMP-9.
  • Demonstrated selective sensor penetration and blocking capabilities through hydrogel mesh size optimization.
  • Validated the clinical feasibility of the FIOL system via in vivo testing.

Conclusions:

  • The novel FIOL-based sensing system provides a promising platform for intraocular fluid liquid biopsy.
  • This technology enables non-invasive monitoring of CNS and eye disorder biomarkers.
  • FIOL represents a significant advancement in implantable diagnostic devices for neurological and ophthalmological conditions.