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Updated: Dec 21, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Repeat RNAs associate with replication forks and post-replicative DNA.

Helene M Gylling1, Cristina Gonzalez-Aguilera1, Martin A Smith2,3

  • 1Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, 2200, Denmark.

RNA (New York, N.Y.)
|May 13, 2020
PubMed
Summary
This summary is machine-generated.

Newly identified repeat-containing RNAs associate with DNA during replication and remain stable throughout the cell cycle, impacting epigenome maintenance. This discovery sheds light on RNA

Keywords:
DNA replicationRNAchromatinrepeats

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genomics

Background:

  • Noncoding RNAs (ncRNAs) regulate chromatin modifications.
  • The role of ncRNA in DNA replication and epigenome maintenance remains unclear.
  • Nascent chromatin contains unknown replication and epigenetic machinery components.

Purpose of the Study:

  • To investigate the role of ncRNA in DNA replication and epigenome maintenance.
  • To characterize RNAs associated with nascent chromatin at replication forks.
  • To track RNA composition during chromatin maturation across the cell cycle.

Main Methods:

  • Isolation and characterization of long and short RNAs from nascent chromatin.
  • Tracking RNA composition during chromatin maturation.
  • Analysis of RNA association with replicated DNA post-replication and across the cell cycle.

Main Results:

  • GA-rich, alpha-satellite, and telomeric repeat-containing RNAs (TERRA) associate with replicated DNA shortly after replication fork passage.
  • These repeat-containing RNAs originate from loci undergoing replication, indicating cis-interaction.
  • Repeat RNAs remain enriched on DNA post-replication, during chromatin maturation, and in G1 phase, suggesting stable association throughout the cell cycle.

Conclusions:

  • Specific repeat RNAs are transcribed post-DNA replication and stably associate with their loci of origin.
  • These findings provide insights into the regulation of repeat RNA-chromatin interactions during DNA replication and the cell cycle.
  • The study presents a method for studying RNA interactions with replication forks and post-replicative chromatin.