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Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Alternative RNA Splicing

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Related Experiment Video

Updated: Dec 21, 2025

Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

Phase separation driven by production of architectural RNA transcripts.

Tetsuya Yamamoto¹, Tomohiro Yamazaki, Tetsuro Hirose

¹Institute for Chemical Reaction Design and Discovery, Hokkaido University, Kita 21 Nishi 10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan. tyamamoto@icredd.hokudai.ac.jp.

|May 13, 2020

Summary

Phase separation driven by architectural RNA (arcRNA) production leads to condensate formation. Condensate size depends on arcRNA production and degradation rates, with most arcRNA degrading within these structures.

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Last Updated: Dec 21, 2025

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Published on: March 25, 2012

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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

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Published on: July 29, 2019

Area of Science:

Biophysics
Molecular Biology
RNA Biology

Background:

Architectural RNA (arcRNA) plays a role in nuclear organization.
Phase separation is a key mechanism for cellular compartmentalization.

Purpose of the Study:

To investigate the role of arcRNA production in driving phase separation using an extended Flory-Huggins theory.
To predict the formation and characteristics of arcRNA-rich condensates.

Main Methods:

Utilized an extension of the Flory-Huggins theory to model arcRNA behavior.
Analyzed diffusion, attractive interactions via proteins, and phase separation dynamics.

Main Results:

Phase separation is driven by constant arcRNA production at a DNA locus.
Condensate radius is determined by the distance from the DNA locus where arcRNA volume fraction jumps.
Condensate volume is proportional to arcRNA production rate and inversely proportional to degradation rate.

Conclusions:

The study predicts condensate formation driven by arcRNA production and segregation.
Most arcRNA molecules are degraded within condensates due to strong segregation.
The findings provide insights into the regulation of nuclear condensates.