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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
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Differentially-Charged Liposomes Interact with Alphaherpesviruses and Interfere with Virus Entry
Oleksandr Kolyvushko1, Juliane Latzke1, Ismail Dahmani2
1Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Street 7-13, 14163 Berlin, Germany.
Pathogens (Basel, Switzerland)
|May 14, 2020
Summary
Charged phospholipids, like phosphatidylserine (PS) and DOTAP, inhibit equine herpesvirus type 1 (EHV-1) infection. These findings suggest charged phospholipids may offer a novel antiviral strategy against EHV-1.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Phosphatidylserine (PS) exposure on the plasma membrane is linked to infections by Alphaherpesvirinae.
- Equine herpesvirus type 1 (EHV-1) may utilize PS during viral entry, but its precise role and that of other phospholipids are unclear.
Purpose of the Study:
- To investigate the interaction between charged phospholipids and EHV-1 particles.
- To determine the influence of these interactions on viral infection.
Main Methods:
- Utilized liposomes with varying phospholipid charges (PS, DOTAP, PC).
- Assessed the effect of these liposomes on EHV-1 infectivity.
- Analyzed the dose and time-dependency of PS-mediated inhibition.
Main Results:
- Negatively charged PS and positively charged DOTAP liposomes inhibited EHV-1 infection.
- Neutral phosphatidylcholine (PC) liposomes had no significant effect on EHV-1 infection.
- PS-mediated inhibition was transient, time-dependent, and dose-dependent.
Conclusions:
- Both cationic and anionic phospholipids interact with EHV-1, reducing infectivity through distinct mechanisms.
- Charged phospholipids demonstrate antiviral potential against EHV-1.
- Charged phospholipids may serve as a basis for developing new EHV-1 antiviral therapies.
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