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Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod
M Monsigny1, C Petit, A C Roche
1Laboratoire de Biochimie Cellulaire et Moléculaire des Glycoconjugués, Centre National de la Recherche Scientifique, Orleans, France.
Analytical Biochemistry
|December 1, 1988
Abstract:
A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.