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Updated: Dec 21, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for
Shayne D Wierbowski1,2, Tommy V Vo2, Pascal Falter-Braun3,4
1Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, NY 14853.
Abstract:
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
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