Related Experiment Video
Updated: Aug 2, 2026
Isolation of Drosophila melanogaster Testes
Published on: May 13, 2011
Modified techniques for the isolation of Dermatophilus spp. from infected material
Abstract:
Two improved techniques are described for the isolation of Dermatophilus spp. The first is suitable for samples of scab from lesions of the disease in animals, except for sheep affected with lumpy wool. The second is for the isolation of the organism from samples of lumpy wool in sheep. Both techniques are simple, rapid and reliable and are based on releasing the organism by pulverizing the scab and then by suppressing contaminants by including polymixin B sulphate in the medium. Excellent results are obtained with or without pre-incubation exposure to CO2.
Insights
Two new methods efficiently isolate Dermatophilus spp. from animal skin lesions and sheep lumpy wool. These techniques are fast, reliable, and use scab pulverization with polymixin B sulphate to reduce contaminants.
Area of Science:
- Veterinary Microbiology
- Dermatophilosis Research
Background:
- Dermatophilus spp. cause significant skin diseases in animals.
- Accurate isolation of Dermatophilus spp. is crucial for diagnosis and control.
- Existing isolation methods can be time-consuming or less effective for specific sample types.
Purpose of the Study:
- To present two improved, rapid, and reliable techniques for isolating Dermatophilus spp.
- To provide effective methods for samples from animal lesions (excluding sheep lumpy wool) and sheep lumpy wool specifically.
Main Methods:
- Technique 1: Pulverization of scab from animal lesions, followed by cultivation on a medium containing polymixin B sulphate.
- Technique 2: Similar isolation from sheep lumpy wool samples.
- Inclusion of polymixin B sulphate in the culture medium to suppress contaminating organisms.
- Evaluation of results with and without pre-incubation exposure to carbon dioxide (CO2).
Main Results:
- Both techniques demonstrated simplicity, speed, and reliability in isolating Dermatophilus spp.
- Excellent results were achieved for both lesion and lumpy wool samples.
- The addition of polymixin B sulphate effectively reduced microbial contamination.
- Pre-incubation with CO2 did not significantly impact the isolation success.
Conclusions:
- The described techniques offer significant improvements for Dermatophilus spp. isolation.
- These methods are practical for routine diagnostic laboratories dealing with dermatophilosis.
- The enhanced isolation protocols contribute to better disease management strategies.
