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Testing Albendazole Resistance in Fasciola hepatica
Luis I Alvarez1, María Martinez Valladares2, Candela Canton3
1Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), UNCPBA-CICPBA-CONICET, Facultad de Ciencias Veterinarias, Campus Universitario, Tandil, Argentina. lalvarez@vet.unicen.edu.ar.
Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2020
Summary
The egg development test detects albendazole resistance in Fasciola hepatica. This simple in vitro method uses parasite eggs from fecal samples, avoiding animal sacrifice.
Area of Science:
- Veterinary Parasitology
- Drug Resistance Studies
Background:
- Albendazole (ABZ) is a key flukicidal drug with ovicidal activity.
- Detecting ABZ resistance in Fasciola hepatica is crucial for effective parasite control.
- Existing methods for resistance detection can be invasive or resource-intensive.
Purpose of the Study:
- To present and validate the egg development test for diagnosing albendazole resistance in Fasciola hepatica.
- To highlight the advantages of this in vitro diagnostic method.
Main Methods:
- Utilizing an in vitro egg development test.
- Assessing the effect of albendazole on egg development and hatching.
- Analyzing eggs isolated from fecal samples of sheep and cattle.
Main Results:
- The test differentiates between ABZ-susceptible and ABZ-resistant Fasciola hepatica isolates.
- Albendazole's ovicidal effect is observed in susceptible isolates but lost in resistant ones.
- The method is applicable to eggs from field samples.
Conclusions:
- The egg development test is a reliable in vitro tool for detecting ABZ resistance in Fasciola hepatica.
- This diagnostic method is simple, inexpensive, and accessible, offering a non-invasive alternative for resistance monitoring.
