Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids01:21

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

348
Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...
348
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents01:29

Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents

381
Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
381
Heart Failure Drugs: Inotropic Agents01:26

Heart Failure Drugs: Inotropic Agents

1.1K
Positive inotropic agents are commonly used as the first line of treatment for heart failure. One such agent is digoxin, derived from the genus Digitalis, which has been known for centuries but effectively utilized since 1785. However, these cardiac glycosides can have potentially toxic effects due to their mechanism of action, which involves inhibiting Na+/K+-ATPase and increasing contractility. Digoxin is absorbed orally and distributed in various tissues, including the CNS. It has a long...
1.1K
Prodrugs01:30

Prodrugs

3.7K
Prodrugs are a class of pharmaceutical compounds that undergo a biotransformation process within the body to be converted into a pharmacologically active drug. Prodrugs are designed to improve the therapeutic properties of the parent drug, such as enhancing bioavailability, increasing stability, or reducing toxicity. The concept of prodrugs revolves around modifying the chemical structure of the original drug to make it more effective or convenient for administration.
Prodrugs help overcome...
3.7K
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids01:25

Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids

1.4K
Inhaled corticosteroids (ICS) are anti-inflammatory drugs used primarily in treating persistent asthma and providing long-term maintenance. They target the bronchial mucosa, the lining of the airways, to control inflammation, a critical factor in asthma progression and exacerbation.
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
1.4K
Drug Biotransformation: Overview01:16

Drug Biotransformation: Overview

3.4K
Pharmaceutical substances known as xenobiotics are predominantly lipophilic and nonionized. This enables them to permeate lipid bilayers, such as cell membranes, and interact with intracellular target receptors. Lipophilic drugs have an advantage in crossing biological barriers and reaching their intended sites of action. However, lipophilic drugs often have a restricted capacity for renal expulsion or elimination from the body. When these drugs enter the kidneys and undergo glomerular...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Total Synthesis of Indigotinoline A and Enabled Stereochemical Revision of Isatindigotindoline B.

Organic letters·2026
Same author

Anti-inflammatory Amide-Alkaloid Dimers from the Roots of <i>Piper nigrum</i> L.: Feature-Guided Discovery and Photocatalytic Synthesis.

Journal of agricultural and food chemistry·2026
Same author

Rapid Discovery of Quassinoids with Antifeedant Activity from <i>Picrasma chinensis</i> Employing Molecular Networking Integrated with Mzmine and DeepMass.

Journal of agricultural and food chemistry·2025
Same author

Rapid Discovery of Guaiane-Type Sesquiterpenoids with Rare Ring Skeletons from <i>Daphne aurantiaca</i> using Molecular Networking Together with MS-DIAL and Self-Built Database.

The Journal of organic chemistry·2025
Same author

Discovery of Diverse Seven-Membered Ring Vibsane Diterpenoids from <i>Viburnum awabuki</i> Utilizing a MassQL-Integrated Strategy and Their Antihepatoma Effect.

Organic letters·2025
Same author

Two new five-membered heterocyclic compounds isolated from <i>Polygonum tinctorium</i>.

Natural product research·2025

Related Experiment Video

Updated: Dec 21, 2025

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
12:04

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Published on: August 14, 2012

29.3K

Highly modified steroids from Inonotus obliquus.

Chun-Xin Zou1, Zi-Lin Hou1, Ming Bai1

  • 1Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. xiaoxiao270@163.com songsj99@163.com.

Organic & Biomolecular Chemistry
|May 14, 2020
PubMed
Summary

Six new steroids were isolated from Inonotus obliquus, including novel 8,14-seco-4-methylpregnanes. Compound 2 demonstrated moderate neuroprotective effects in human neuroblastoma cells, highlighting potential therapeutic applications.

More Related Videos

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
08:15

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Published on: December 9, 2022

2.1K
Author Spotlight: Developing a Rat Model for Weight-Bearing Intervention to Investigate Osteonecrosis of the Femoral Head
05:55

Author Spotlight: Developing a Rat Model for Weight-Bearing Intervention to Investigate Osteonecrosis of the Femoral Head

Published on: September 27, 2024

850

Related Experiment Videos

Last Updated: Dec 21, 2025

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
12:04

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Published on: August 14, 2012

29.3K
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
08:15

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Published on: December 9, 2022

2.1K
Author Spotlight: Developing a Rat Model for Weight-Bearing Intervention to Investigate Osteonecrosis of the Femoral Head
05:55

Author Spotlight: Developing a Rat Model for Weight-Bearing Intervention to Investigate Osteonecrosis of the Femoral Head

Published on: September 27, 2024

850

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Mycology

Background:

  • The fungus Inonotus obliquus is a source of bioactive compounds.
  • Steroids represent a class of molecules with diverse pharmacological properties.

Purpose of the Study:

  • To isolate and characterize novel steroid compounds from Inonotus obliquus.
  • To evaluate the neuroprotective potential of these isolated steroids.

Main Methods:

  • Isolation and purification of compounds using chromatographic techniques.
  • Structure elucidation employing spectroscopic data analyses (NMR, MS), quantum chemical calculations, and DP4+ probability analysis.
  • Determination of absolute configurations via electronic circular dichroism (ECD) spectroscopy and single-crystal X-ray diffraction for compound 1.
  • Assessment of neuroprotective activity using an in vitro model of hydrogen peroxide-induced oxidative stress in SH-SY5Y cells.

Main Results:

  • Six previously undescribed steroids were isolated from Inonotus obliquus.
  • Compounds 1 and 2 are the first reported examples of the 8,14-seco-4-methylpregnane skeleton.
  • The structures and absolute configurations of all six compounds were unambiguously determined.
  • Compound 2 exhibited moderate neuroprotective activity against H2O2-induced cell injury in SH-SY5Y cells at a concentration of 12.5 μM.

Conclusions:

  • The study successfully identified novel steroids from Inonotus obliquus, expanding the known chemical diversity of this fungus.
  • The discovery of the 8,14-seco-4-methylpregnane class provides new structural motifs for drug discovery.
  • Compound 2 shows promise as a neuroprotective agent, warranting further investigation for therapeutic development.