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Updated: Dec 21, 2025

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
Published on: August 14, 2012
Highly modified steroids from Inonotus obliquus
Chun-Xin Zou1, Zi-Lin Hou1, Ming Bai1
1Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. xiaoxiao270@163.com songsj99@163.com.
Abstract:
Six undescribed steroids were isolated from the fungus Inonotus obliquus. Notably, compounds 1 and 2 represented the first example of 8,14-seco-4-methylpregnane. By spectroscopic data analyses, quantum chemical calculations and DP4+ probability analysis, their structures were unambiguously determined. The absolute configurations of the compounds 1-6 were defined by comparison of their experimental and calculated electronic circular dichroism (ECD) spectra data. The structure of compound 1 was also confirmed by single-crystal X-ray diffraction. All isolated steroids were evaluated for their neuroprotective activities against H2O2-induced cell injury in human neuroblastoma SH-SY5Y cells, among which compound 2 showed moderate activity at 12.5 μM.
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