Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation01:22

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

702
Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
702
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

205
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
205
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

403
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
403

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gadolinium retention in rat abdominal organs after administration of gadoxetic acid disodium compared to gadodiamide and gadobutrol.

Magnetic resonance in medicine·2020
Same author

Pre-steady state kinetic analysis of HIV-1 reverse transcriptase for non-canonical ribonucleoside triphosphate incorporation and DNA synthesis from ribonucleoside-containing DNA template.

Antiviral research·2015
Same author

Combined lateral femoral epicondylar osteotomy and a submeniscal approach for the treatment of a tibial plateau fracture involving the posterolateral quadrant.

Injury·2014
Same author

Effects of a continuous electromagnetic field on wound healing in human airway.

The Laryngoscope·2014
Same author

Potent trypanocidal curcumin analogs bearing a monoenone linker motif act on trypanosoma brucei by forming an adduct with trypanothione.

Molecular pharmacology·2014
Same author

Kinetic variations between reverse transcriptases of viral protein X coding and noncoding lentiviruses.

Retrovirology·2014

Related Experiment Video

Updated: Dec 21, 2025

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

9.9K

L-glutamine as a T2 exchange contrast agent.

Chan Gyu Joo1, Seung-Hyun Yang2, Yuna Choi2

  • 1Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul, Korea.

Magnetic Resonance in Medicine
|May 15, 2020
PubMed
Summary

L-glutamine shows potential as a T2 exchange contrast agent for Magnetic Resonance Imaging (MRI). This study demonstrates its ability to visualize glutamine uptake in vivo within tumors.

Keywords:
cancer imagingexchange contrast agentglutamine

More Related Videos

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.6K
Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
06:32

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

2.0K

Related Experiment Videos

Last Updated: Dec 21, 2025

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

9.9K
MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.6K
Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
06:32

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

2.0K

Area of Science:

  • Biophysics
  • Medical Imaging
  • Biochemistry

Background:

  • Magnetic Resonance Imaging (MRI) relies on contrast agents to enhance image clarity.
  • Investigating novel agents like L-glutamine can expand MRI's diagnostic capabilities.
  • Understanding amino acid metabolism is crucial for cancer research.

Purpose of the Study:

  • To evaluate L-glutamine as a T2 exchange contrast agent in MRI.
  • To assess the feasibility of imaging L-glutamine uptake in vivo.

Main Methods:

  • Measured T2 relaxation rates of L-glutamine solutions at various concentrations and 9.4 T.
  • Acquired T2-weighted MRI images in a mouse cancer model following L-glutamine infusion.
  • Quantified T2 relaxivity (R2ex) attributed to exchange processes.

Main Results:

  • L-glutamine exhibited T2 relaxivity (R2ex) of 0.069 s⁻¹ mM⁻¹ at 37°C and pH 7.2.
  • Glutamine R2ex values ranged from 0.097-0.1 s⁻¹ mM⁻¹ at pH 6.1-6.7.
  • In vivo MRI demonstrated localized glutamine uptake within tumors.

Conclusions:

  • L-glutamine functions effectively as a T2 exchange contrast agent.
  • MRI can successfully visualize L-glutamine uptake in living organisms.
  • This technique holds promise for studying tumor metabolism and drug delivery.