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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Dec 21, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

A highly sensitive bioluminescent method for measuring allergen-specific IgE in microliter samples

Sophie Goyard¹, Bianca Balbino^2,3, Rebecca S Chinthrajah^4,5

¹Unit of Lymphocyte Cell Biology, U1221 INSERM, Institut Pasteur, Paris, France.

|May 15, 2020

Summary

No abstract available in PubMed .

Keywords:

IgE allergy diagnosis bioluminescence luciferase nanobody

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