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When conducting an experiment, it is crucial to have control to reduce bias and accurately measure the dependent variables. It also marks the results more reliable. Controls are elements in an experiment that have the same characteristics as the treatment groups but are not affected by the independent variable. By sorting these data into control and experimental conditions, the relationship between the dependent and independent variables can be drawn. A randomized experiment always includes a...
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Related Experiment Video

Updated: Dec 21, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
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COVID-19 Follow up Testing.

Amelita Woodruff1

  • 1Mayo Clinic, Department of Family Medicine, 4500 San Pablo Rd., Jacksonville, FL 32224, United States.

The Journal of Infection
|May 15, 2020
PubMed
Summary
This summary is machine-generated.

Many SARS-CoV-2 positive patients continued to test positive even after meeting CDC quarantine release guidelines. The average time to achieve a negative test result after symptom onset was 19 days.

Keywords:
COVID-19CoronavirusInfection ControlPolymerase chain reactionSARS-CoV-2StatisticsTestingViral infection

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Area of Science:

  • Infectious Diseases
  • Virology
  • Public Health

Background:

  • SARS-CoV-2, the virus causing COVID-19, led to significant public health challenges.
  • Understanding viral shedding and transmission is crucial for effective public health strategies.
  • The Mayo Clinic FL COVID Virtual Clinic monitored patients during the pandemic.

Purpose of the Study:

  • To analyze SARS-CoV-2 positive cases within the Mayo Clinic FL COVID Virtual Clinic.
  • To evaluate the duration of viral positivity in relation to Centers for Disease Control and Prevention (CDC) quarantine guidelines.
  • To determine the average time from symptom onset to negative SARS-CoV-2 testing.

Main Methods:

  • Retrospective analysis of SARS-CoV-2 positive cases.
  • Assessment of patient data against CDC quarantine release criteria.
  • Tracking of time from symptom onset to achieving negative test results.

Main Results:

  • SARS-CoV-2 positive cases were identified within the virtual clinic.
  • A significant proportion (70%) of patients met CDC guidelines for release from quarantine but continued to test positive.
  • The average duration from symptom onset to a negative SARS-CoV-2 test was 19 days.

Conclusions:

  • Current CDC quarantine release guidelines may not fully align with the duration of viral shedding for all SARS-CoV-2 positive individuals.
  • Extended periods of positivity beyond recommended quarantine durations highlight the need for further research into viral clearance.
  • These findings have implications for public health policy and patient management strategies during infectious disease outbreaks.