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Propionate and the production of monensins in Streptomyces cinnamonensis
J Matĕjů1, J Karnetová, M Nohýnek
1Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Abstract:
Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.
Insights
Researchers developed propionate-resistant Streptomyces cinnamonensis variants to study monensin production. Most variants showed stimulated monensin synthesis when exposed to higher propionate levels, indicating propionate
Area of Science:
- Microbiology
- Biotechnology
- Fermentation Technology
Background:
- Streptomyces cinnamonensis is a key producer of monensin, an ionophore antibiotic.
- Monensin production is influenced by media components, including propionate.
- Understanding regulatory mechanisms of monensin biosynthesis is crucial for optimizing production.
Purpose of the Study:
- To investigate the effect of propionate resistance on monensin production in Streptomyces cinnamonensis.
- To determine if propionate can stimulate monensin synthesis in resistant strains.
- To analyze the impact of propionate on the ratio of monensin A to monensin B.
Main Methods:
- Preparation of propionate-resistant mutants from Streptomyces cinnamonensis.
- Cultivation of selected resistant variants in media with varying propionate concentrations.
- Quantification of monensin production (monensin A and B) using analytical techniques.
Main Results:
- Seventy percent of the propionate-resistant variants exhibited stimulated monensin synthesis.
- Higher propionate concentrations in the medium enhanced monensin production in responsive resistant strains.
- The ratio of monensin A to monensin B production remained unaffected by propionate.
Conclusions:
- Propionate plays a significant role in stimulating monensin biosynthesis in Streptomyces cinnamonensis.
- Developing resistant strains can be a viable strategy to enhance monensin yields.
- The regulation of monensin A and B production ratios is independent of propionate stimulation.