Related Experiment Video

Updated: Dec 21, 2025

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

4.1K

miR-487a performs oncogenic functions in osteosarcoma by targeting BTG2 mRNA

Zhiqian Gu1,2, Shaokun Wu1,2, Guoxing Xu3

  • 1Department of Orthopedics, Hwa Mei Hospital, University of Chinese Academy of Sciences (Ningbo No. 2 Hospital), Ningbo 315000, China.

Acta Biochimica Et Biophysica Sinica
|May 16, 2020
PubMed

Insights

MicroRNA-487a (miR-487a) is elevated in osteosarcoma, promoting tumor growth and invasion by downregulating B-cell translocation gene 2 (BTG2). Inhibiting miR-487a suppressed tumor progression in preclinical models.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in osteosarcoma (OS) development.
  • Understanding specific miRNA roles is crucial for OS pathogenesis research.

Purpose of the Study:

  • To investigate the role of microRNA-487a (miR-487a) in osteosarcoma.
  • To elucidate the molecular mechanisms by which miR-487a influences OS progression.

Main Methods:

  • Analysis of miR-487a expression in OS clinical samples and cell lines.
  • In vitro functional assays (cell growth, invasion, apoptosis) following miR-487a manipulation.
  • In vivo studies using OS xenografts.
  • Identification and validation of miR-487a targets using techniques like small interfering RNA (siRNA).

Main Results:

  • miR-487a was significantly upregulated in OS tissues and cells.
  • miR-487a knockdown inhibited OS cell proliferation, invasion, and tumor growth in vivo, while inducing apoptosis.
  • miR-487a overexpression promoted OS cell growth and invasion.
  • B-cell translocation gene 2 (BTG2) was identified as a direct target of miR-487a.
  • BTG2 knockdown mimicked miR-487a's oncogenic effects, and BTG2 overexpression partially reversed them.
  • A negative correlation between miR-487a and BTG2 expression was observed in OS samples.

Conclusions:

  • miR-487a acts as an oncogenic miRNA in osteosarcoma.
  • miR-487a promotes OS progression by suppressing the expression of its target gene, BTG2.
  • Targeting miR-487a represents a potential therapeutic strategy for osteosarcoma.
Keywords:
BTG2invasionmiR-487aosteosarcomaproliferation

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.8K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.6K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.0K