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Updated: Dec 21, 2025
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Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
Published on: August 20, 2013
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Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform
Ruofan Yu1,2, Myeong Chan Jo3, Weiwei Dang4,5
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2020
Summary
Budding yeast replicative aging (RLS) studies are essential for understanding molecular aging mechanisms. This research details an automated microfluidic system for high-throughput RLS measurement, improving efficiency and data collection.
Area of Science:
- Gerontology
- Cell Biology
- Microbiology
Background:
- Budding yeast, Saccharomyces cerevisiae, serves as a model organism for aging research.
- Traditional replicative lifespan (RLS) measurement is labor-intensive, involving manual cell dissection.
- Automated microfluidic systems have emerged to accelerate RLS determination.
Purpose of the Study:
- To present a detailed protocol for measuring yeast replicative lifespan (RLS).
- To demonstrate the utility of a commercially available microfluidic system for high-throughput RLS analysis.
Main Methods:
- Utilizing a microfluidic system with the HYAA chip design for automated cell dissection.
- Culturing and monitoring Saccharomyces cerevisiae cells to determine RLS.
- Collecting data from a large number of cells simultaneously.
Main Results:
- The described protocol enables efficient and automated RLS measurement.
- The microfluidic system allows for data collection from up to 8000 cells per experiment.
- Significant acceleration of RLS determination compared to manual methods.
Conclusions:
- Automated microfluidic systems, like the one detailed, streamline aging research in yeast.
- This protocol facilitates high-throughput analysis of replicative aging, advancing the study of molecular aging mechanisms.
