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Updated: Dec 21, 2025
The Treadmill Fatigue Test: A Simple, High-throughput Assay of Fatigue-like Behavior for the Mouse
Published on: May 31, 2016
Mouse Fitness as Determined Through Treadmill Running and Walking
Joseph C Reynolds1, Changhan Lee2,3,4
1Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
None:
Endurance testing simultaneously assesses a wide variety of physiological systems including the cardiovascular, respiratory, metabolic, and neuromuscular systems (Gabriel and Zierath, Cell Metab 25:1000-1011, 2017). Treadmill running is a noninvasive method to evaluate fitness capacity in a longitudinal or cross-sectional manner. High-intensity exercise tests can be used to determine peak physical capacity in mice. However, because aging is associated with a progressive loss of physical capacity the running protocols can be adapted and optimized for aged mice.
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