Separation of Friend erythroleukaemic cell histones and high-mobility-group proteins by reversed-phase

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H Lindner1, W Helliger, B Puschendorf

  • 1Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria.

Journal of Chromatography
|October 26, 1988
PubMed

A procedure for the rapid separation of histones and high-mobility-group (HMG) proteins from Friend erythroleukaemic cells (line F4N) by reversed-phase high-performance liquid chromatography is reported. By using a Nucleosil 300-5 C4 column and a multistep water-acetonitrile gradient containing 0.1% trifluoroacetic acid, the HMG-1 and HMG-2 proteins, several H1 subfractions including H1(0), H4, H2B, two H2A variants and two H3 subfractions were separated. Under changed conditions, by applying a varied acetonitrile gradient system, even two H2B variants were fractionated. The methods described seem to be a real alternative to the time-consuming polyacrylamide gel electrophoresis.

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