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A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells
Jing Bai1,2, Haojie Fu1, Lauren Bazinet1
1The Vascular Biology Program and Department of Surgery, Boston Children's Hospital, Harvard Medical School, Boston, MA, United States.
Frontiers in Pharmacology
|May 16, 2020
Summary
Researchers developed a novel 3D micro-engineered cornea using primary cells. This organ-on-a-chip model enhances corneal research and ocular drug permeation studies.
Area of Science:
- Biomedical Engineering
- Ophthalmology
- Drug Delivery Systems
Background:
- Organ-on-a-chip technology offers advanced models for biological research.
- Current corneal models have limitations in recapitulating in vivo functions and drug testing.
Purpose of the Study:
- To develop a primary cell-based corneal microphysiological system.
- To create a 3D micro-engineered cornea for studying corneal functions and ocular drug permeation.
Main Methods:
- Optimized protocols for isolating and cultivating primary corneal epithelial and endothelial cells from C57BL/6J mice.
- Established a microfluidic system for a 3D micro-engineered cornea model.
Main Results:
- Successfully developed a primary cell-based microfluidic corneal model.
- The 3D model overcomes limitations of 2D cell cultures for corneal research.
Conclusions:
- The novel corneal microphysiological system supports advanced investigations into cornea function.
- This platform facilitates effective drug delivery testing for ocular applications.
