Detection of low molecular kininogen messenger RNA in human kidney

The purpose of the present study was to confirm expression of kininogen in human kidneys. As the human kininogen gene is alternatively transcribed to high molecular weight and low molecular weight kininogen messenger (m)RNA, we constructed a probe which can discern these two transcripts by the S1 nuclease mapping analysis. The low molecular weight kininogen mRNA was detected in human kidney cortex and medulla. This finding strongly supports the existence of a local kinin-kallikrein system in human kidneys.